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ARTN Antibody (Biotin)

SKU: orb1272788

Description

ARTN Antibody (Biotin)

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
ELISA:Sandwich:To detect hArtemin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required.Western Blot:To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hArtemin. Human Artemin specific antibody was purified by affinity chromatography and then biotinylated.
TargetARTN
PurificationArtemin antibody was purified by affinity chromatography and then biotinylated.
ConjugationBiotin

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
DisclaimerFor research use only

Alternative Names

EVN, NBN, ENOVIN, EVN, Artemin, Enovin,

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ARTN Antibody (Biotin)

To detect hArtemin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Artemin (orb1272792) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hArtemin.

ARTN Antibody (Biotin)

To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ARTN Antibody (Biotin)

To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ5T4W7

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ARTN Antibody (Biotin) (orb1272788)

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0.05 mg
$ 780.00
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