ARPC1A Antibody

SKU: orb1248739

Description

ARPC1A Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DFKTLESSIQGLR
• Target: ARPC1A
• Molecular Weight: Approx 45 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARPC1A, actin related protein 2/3 complex, subunit 1A, 41kDa, Arc40, SOP2Hs, SOP2L, OTTHUMP00000206034, SOP2-like protein, actin binding protein (Schizosaccharomyces pombe sop2-like), actin-related protein 2/3 complex subunit 1A

orb1248739 (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248739 (0.1 ug/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248739 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ARPC1A

UniProt

Q92747

RefSeq (Protein):NP_001177925.1, NP_006400.2