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Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated
Catalog Number: orb348065
| Catalog Number | orb348065 |
|---|---|
| Category | Antibodies |
| Description | Armenian Hamster IgG (H&L) antibody (Alkaline Phosphatase) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Isotype | IgG |
| Conjugation | AP |
| Reactivity | Hamster |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum. Diminished reactivity will occur with Golden Syrian Hamster IgG. |
| Immunogen | Armenian Hamster IgG whole molecule |
| Tested applications | DOT, ELISA, IHC, WB |
| Dilution range | ELISA: 1:20,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Application notes | Hamster Secondary Antibody conjugated to alkaline phosphatase is available in a variety of formats. Anti-Armenian Hamster IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Alternative names | goat anti-Armenian Hamster IgG Antibody alkaline p Read more... |
| Note | For research use only |
ELISA Results of Goat Anti-Armenian Hamster IgG Antibody Alkaline Phosphatase Conjugate tested against purified Armenian Hamster IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n orb346446). The working dilution is 1:4000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.
