Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

SKU: orb348065

Description

Armenian Hamster IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Hamster
• Application Notes:
  Hamster Secondary Antibody conjugated to alkaline phosphatase is available in a variety of formats. Anti-Armenian Hamster IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Armenian Hamster IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum. Diminished reactivity will occur with Golden Syrian Hamster IgG.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Armenian Hamster IgG Antibody alkaline phosphatase Conjugation, goat anti-Armenian Hamster IgG alkaline phosphatase Conjugated Antibody, goat Anti-Hamster IgG alk phos Conjugated Secondary Antibody

ELISA Results of Goat Anti-Armenian Hamster IgG Antibody Alkaline Phosphatase Conjugate tested against purified Armenian Hamster IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n orb346446). The working dilution is 1:4000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.