ARID3A Antibody

• Catalog Number: orb1256264
• Category: Antibodies
• Description: ARID3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 95kDa
• Target: ARID3A
• UniProt ID: Q99856
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ARID3A antibody (orb1256264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug ARID3A antibody (orb1256264). Western blot was performed from the immunoprecipitate using ARID3A antibody (orb1256264) at a dilution of 1:500.