ARID3A Antibody

SKU: orb1256264

Description

ARID3A Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
• Target: ARID3A
• Molecular Weight: Observed: 95kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1

Western blot analysis of extracts of various cell lines, using ARID3A antibody (orb1256264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (orb1256264) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug ARID3A antibody (orb1256264). Western blot was performed from the immunoprecipitate using ARID3A antibody (orb1256264) at a dilution of 1:500.

Quick Database Links

Gene Symbol

ARID3A

UniProt

Q99856