ARHGAP1 Antibody

• Catalog Number: orb1257835
• Category: Antibodies
• Description: ARHGAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 50kDa
• Target: ARHGAP1
• UniProt ID: Q07960
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Rho GTPase-activating protein 1, CDC42 GTPase-acti
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ARHGAP1 antibody (orb1257835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunofluorescence analysis of HeLa cells using ARHGAP1 antibody (orb1257835) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug ARHGAP1 antibody (orb1257835). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (orb1257835) at a dilution of 1:1000.