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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:100-100, IP: 1:20-50 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 47-115 of human Argonaute-2 (NP_036286.2). |
| Molecular Weight | 110kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AGO2, CASC7, EIF2C2, LINC00980, PPD, Q10, protein argonaute-2, Argonaute 2, EIF2C2
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Immunofluorescence analysis of L929 cells using Argonaute-2 Polyclonal Antibody (orb373479) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Argonaute-2 antibody (orb373479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Zhang, Lili et al. LncRNA ORLNC1 Promotes Bone Marrow Mesenchyml Stem Cell Pyroptosis Induced by Advanced Glycation End Production by Targeting miR-200b-3p/Foxo3 Pathway Stem Cell Rev Rep, (2021)
Argonaute-2 Rabbit pAb (orb373479)
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