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ARFGAP1 Antibody (C-term)
Catalog Number: orb1931647
| Catalog Number | orb1931647 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the C-terminal region of human ARFGAP1. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | Q8N6T3 |
| MW | 44668 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:8000, WB: 1:1000, IHC-P: 1:50~100 |
| Antibody Type | Primary Antibody |
| Clone Number | RB4855 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | ADP-ribosylation factor GTPase-activating protein Read more... |
| Research Area | Cell Biology, Signal Transduction |
| Note | For research use only |
| NCBI | NP_060679.1, NP_001268413.1, NP_001268411.1, NP_001268412.1, NP_783202.1 |
| Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of ARFGAP1 Antibody (C-term) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right). ARFGAP1 (arrow) was detected using the purified Pab.
All lanes: Anti-ARFGAP1 Antibody (C-term) at 1: 8000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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