ARFGAP1 Antibody (C-term)

• Catalog Number: orb1931647
• Category: Antibodies
• Description: ARFGAP1 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q8N6T3
• MW: 44668 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ARF1GAP
• Research Area: Cell Biology, Signal Transduction
• Note: For research use only

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of ARFGAP1 Antibody (C-term) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right). ARFGAP1 (arrow) was detected using the purified Pab.

All lanes: Anti-ARFGAP1 Antibody (C-term) at 1: 8000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.