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ARF GAP1 rabbit pAb

Catalog Number: orb769628

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb769628
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ARF GAP1
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityBovine, Human, Monkey, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human ARFGAP1. AA range:171-220
MW48
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-ARFGAP1 antibody, anti-ARF1GAP antibody, anti
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NoteFor research use only
ARF GAP1 rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

ARF GAP1 rabbit pAb

Immunofluorescence analysis of MCF7 cells, using ARFGAP1 Antibody. The picture on the right is blocked with the synthesized peptide.

ARF GAP1 rabbit pAb

Western blot analysis of lysates from COS7 cells, using ARFGAP1 Antibody. The lane on the right is blocked with the synthesized peptide.