Aquaporin 3 Antibody

SKU: orb67573

Description

Rabbit Anti-Rat Aquaporin 3 Polyclonal

Research Area

Kidney & Urological Research

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:2000), IHC-P (1:200), ICC/IF (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
• Target: Aquaporin 3
• Molecular Weight: 35-50kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aquaporin 3, Aquaporin-3, AQP3, AQP-3, AQP 3, Aqp3, AQP3_HUMAN, Aquaglyceroporin-3, Aquaporin 3 (GIL blood group), Aquaporin 3 (Gill blood group), Aquaporin3, GIL, Gill blood group

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 3 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 3 protein using Rabbit Anti-Aquaporin 3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody at 1:2000.

Quick Database Links

Gene Symbol

Aquaporin 3

UniProt

P47862

NCBI Gene ID

65133

RefSeq (Protein):NP_113891.1