Aquaporin 3 Antibody (FITC)

SKU: orb152820

Description

Rabbit polyclonal antibody against Aquaporin 3 Conjugated to FITC

Research Area

Cell Biology, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB (1:2000), IHC (1:200), ICC/IF (1:400)
Reactivity	Human, Mouse, Rat
Application Notes	0.5 µg/ml was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Target	Aquaporin 3
Molecular Weight	35-50kDa
Purification	Protein A Purified
Conjugation	FITC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Aquaporin 3, Aquaporin-3, AQP3, AQP-3, AQP 3, Aqp3, AQP3_HUMAN, Aquaglyceroporin-3, Aquaporin 3 (GIL blood group), Aquaporin 3 (Gill blood group), Aquaporin3, GIL, Gill blood group

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Immunohistochemistry analysis using Rabbit Anti-Aquaporin 3 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 3 protein using Rabbit Anti-Aquaporin 3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody at 1:2000.

Quick Database Links

Gene Symbol

Aquaporin 3

UniProt

P47862

NCBI Gene ID

65133

RefSeq (Protein):NP_113891.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_113891.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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