Aquaporin 2 Antibody (FITC)

SKU: orb152803

Description

Rabbit polyclonal antibody against Aquaporin 2 Conjugated to FITC

Images & Validation

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Tested Applications	ICC, IF, IHC, WB
Dilution range	WB (1:2000), IHC (1:200), ICC/IF (1:400)
Reactivity	Human, Mouse, Rat
Application Notes	0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
Target	Aquaporin 2
Molecular Weight	35-50kDa
Purification	Protein A Purified
Conjugation	FITC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Aquaporin 2, Aquaporin-2, AQP2, AQP-2, AQP 2, AQP-CD, AQP CD, AQPCD, AQP2_HUMAN, Aquaporin 2 collecting duct, Aquaporin CD, Aquaporin-CD, Aquaporin2, Aquaporine 2, ADH water channel, Collecting duct water channel protein, MGC34501, Water channel aquaporin 2, Water channel protein for renal collecting duct, WCH CD, WCH-CD, WCHCD

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Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody at 1:2000. Showing glycosylated and non-glycosylated bands.

Quick Database Links

Gene Symbol

Aquaporin 2

UniProt

P34080

NCBI Gene ID

25386

RefSeq (Protein):NP_037041.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_037041.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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