Aquaporin 1 Antibody (Biotin)

SKU: orb152785

Description

Rabbit polyclonal antibody against Aquaporin 1 Conjugated to Biotin

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:2000), IHC (1:200), ICC/IF (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1
• Target: Aquaporin 1
• Molecular Weight: 35-50kDa
• Purification: Protein A Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Aquaporin 1, Aquaporin-1, AQP1, AQP-1, AQP 1, AQP CHIP, AQP1_HUMAN, Aquaporin CHIP, Aquaporin-CHIP, Aquaporin1, Channel forming integral protein 28kDa, Channel-like integral membrane protein 28 kDa, CHIP 28, CHIP28, CO, Colton blood group, Growth factor induced delayed early response protein, MGC26324, Urine water channel, Water channel protein CHIP 29, Water channel protein CHIP29, Water channel protein for red blood cells and kidney proximal tubule

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:2000. Showing glycosylated and non-glycosylated bands.

Quick Database Links

Gene Symbol

Aquaporin 1

UniProt

P29975

NCBI Gene ID

25240

RefSeq (Protein):NP_036910.1