Aquaporin 1 Antibody: Biotin

• Catalog Number: orb152785
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Aquaporin 1 Conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), IHC (1:200), ICC/IF (1:400)
• Conjugation: Biotin
• MW: 35-50kDa
• Target: Aquaporin 1
• Entrez: 25240
• UniProt ID: P29975
• NCBI: NP_036910.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: AQP1 antibody, AQP 1 antibody, AQP CHIP antibody,
• Note: For research use only
• Application notes: 0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:2000. Showing glycosylated and non-glycosylated bands.