APRT Antibody (N-term)

• Catalog Number: orb1788008
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human APRT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: 27-60 aa
• UniProt ID: P07741
• MW: 19608 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Adenine phosphoribosyltransferase, APRT, APRT
• Note: For research use only

Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-APRT Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: A2058 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cell stained with APRT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.