APP/Beta Amyloid Precursor Antibody

SKU: orb1537949

Description

APP/Beta Amyloid Precursor Antibody

Research Area

Neuroscience; Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA, IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone No.: J4H9
• Immunogen: Recombinant human APP (18-289aa) purified from E. coli.
• Target: APP / Beta Amyloid Precursor
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APP, ABPP, A4, ABETA, AD1, Alzheimer disease, Amyloid beta, Beta-amyloid peptide, APPI, Beta amyloid, CVAP, AAA, PN2, PreA4, Amyloid beta A4 protein, Peptidase nexin-II, CTFgamma, PN-II, Protease nexin-II

WB analysis of orb1537949.

Quick Database Links

Gene Symbol

APP / Beta Amyloid Precursor