APP Antibody

SKU: orb1239206

Description

APP Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Guinea pig, Porcine
• Application Notes:
  APP antibody can be used for detection of APP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: APP antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APP.The immunogen is located within amino acids 180 - 230 of APP.
• Target: APP
• Purification: APP Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: APP Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP

Western blot analysis of APP in rat brain tissue lysate with APP antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 µg/mL.

Immunofluorescence of APP in mouse brain tissue with APP antibody at 20 µg/mL.

Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 µg/mL.

Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 µg/mL. Green: APP antibody (orb1239206) Red: Phylloidin staining Blue: DAPI staining.

Quick Database Links

Gene Symbol

APP

UniProt

P05067

RefSeq:CAA30050