APP Antibody

• Catalog Number: orb1239195
• Category: Antibodies
• Description: APP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Guinea pig, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.The immunogen is located within the last 50 amino acids of APP.
• Concentration: 1 mg/ml
• Dilution range: APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 83 kDa Observed: 110 kDa
• Target: APP
• UniProt ID: P05067
• NCBI: CAA30050
• Storage: APP antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: APP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABE
• Note: For research use only
• Application notes: APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 µg/ml.

Immunohistochemical staining of rat brain using APP antibody at 2 µg/mL.

Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 µg/mL.

Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 µg/mL.

Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 µg/mL. Green: APP antibody (orb1239195) Red: Phylloidin staining Blue: DAPI staining.