APP Antibody

• Catalog Number: orb1239089
• Category: Antibodies
• Description: APP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Predicted Reactivity: Bovine, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases.The immunogen is located within amino acids 650 - 700 of APP.
• Concentration: 1 mg/mL
• Dilution range: APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 83 kDa Observed: 115 kDa
• Target: APP
• UniProt ID: P05067
• NCBI: CAA30050
• Storage: APP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: APP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABE
• Note: For research use only
• Application notes: APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of APP in (A) human, (B) mouse, and (C) rat brain tissue lysates with APP antibody at 1 µg/mL.

Immunohistochemistry of APP in human brain (Alzheimer's disease) tissue with APP antibody at 10 µg/mL.

Immunohistochemistry of APP in human brain tissue with APP antibody at 2.5 µg/ml.

Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 µg/mL. Green: APP Antibody (orb1239089) Blue: DAPI staining.