APP Antibody (N-term)

• Catalog Number: orb1930103
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5012
• Tested applications: FC, IHC-P, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 86943 Da
• Target: This APP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-61 amino acids from the N-terminal region of human APP.
• UniProt ID: P05067
• NCBI: NP_000475.1, NP_958816.1, NP_001191232.1, NP_001129601.1, NP_001129602.1, NP_001129488.1, NP_958817.1, NP_001191230.1, NP_001191231.1, NP_001129603.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Amyloid beta A4 protein, ABPP, APPI, APP, Alzheime
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HepG2 cells using APP Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain with APP Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-hAPP-H44 at 1:1000 dilution + Mouse brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.