Apoptosis Research Antibody Assembly kit(bcl-2, bax, Caspase-3, Caspase-9, Cytochrome C)

SKU: orb1012329

Description

Apoptosis Research Antibody Assembly kit(bcl-2, bax, Caspase-3, Caspase-9, Cytochrome C)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB=1:500-5000, IHC-P=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Primary: Anti-Caspase 8 at 1/300 dilution, Sample: Thymus (Mouse) Lysate at 40 ug, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12, 55 kD, Observed band size: 17 kD.

Primary: Anti-Cytochrome C at 1/1000 dilution Sample: Liver (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/26 kD Observed band size: 12.8 kD.

Primary: Anti-Bax at 1/1000 dilution Sample: 293T (Human) Cell Lysate at 30 ug 293T-UV (Human) Cell Lysate at 30 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD.

Primary: Anti-Caspase 3 precursor at 1/1000 dilution, Sample: Spleen (Mouse) Lysate at 40 ug, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Primary: Anti-Caspase-9 at 1/300 dilution, Sample: K562 Cell (Human) Lysate at 40 ug, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35/50 kD, Observed band size: 35 kD.