APOLIPOPROTEIN C-III Antibody

SKU: orb345280

Description

APOLIPOPROTEINC-III antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:10,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Apolipoprotein antibodies have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: produced from apoLipoprotein Type C-III derived from human plasma
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Apolipoprotein C-III antibody, ApoC-III, Apo-CIII antibody, Apolipoprotein C3, Apolipoprotein C III antibody

Immunohistochemistry of goat anti-Apolipoprotein C-III antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-III at 2.5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10000 for 45 min at RT. Staining: Apolipoprotein C-III as precipitated red signal with hematoxylin purple nuclear counterstain.

Quick Database Links

UniProt

P02656

RefSeq:CAA25648.1