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| Catalog Number | orb764557 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ApoL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APOL1. AA range:261-310 |
| MW | 44 |
| Tested applications | ELISA, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-APOL1 antibody, anti-APOL antibody, anti-Apol Read more... |
| Note | For research use only |
Western Blot analysis of various cells using ApoL1 Polyclonal Antibody diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from A549 cells, using APOL1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from K562 cells using APOL1 antibody.
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