APOE/Apolipoprotein E Antibody

• Catalog Number: orb1535052
• Category: Antibodies
• Description: APOE/Apolipoprotein E Antibody
• Target: APOE / Apolipoprotein E
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 19 AA peptide near the C-Terminus of human APO-E. The immunogen is located within aa 220-270 of APO-E.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF, IHC, IHC-P (10 µg/ml), WB (0.5 - 2 µg/ml)
• Application notes: Further information: APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 35 kDa Observed: 34 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APOE, AD2, Apo-E, Apolipoprotein E3, Apolipoprotei
• Note: For research use only

Anti-APOE / Apolipoprotein E antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence of APO E in mouse brain tissue with APO E Antibodyat 20 µg/mL. Green: APO E antibody Red: Phylloidin staining Blue: DAPI staining

Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 ug/ml.