APOE Antibody (Biotin)

SKU: orb1272734

Description

APOE Antibody (Biotin)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hApoE3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human ApoE3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Western Blot:To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography and then biotinylated. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA
• Target: APOE
• Conjugation: Biotin

Storage & Handling

• Storage: ApoE3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AD2, LPG, LDLCQ5Apolipoprotein EApo-E

To detect hApoE3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human ApoE3 (orb1272735) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hApoE3.

Quick Database Links

Gene Symbol

APOE

UniProt

P02649

RefSeq:P02649