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APOB Antibody / Apolipoprotein B
Catalog Number: orb1151765
| Catalog Number | orb1151765 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to APOB |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG1, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | A portin of amino acids 592-689 from the human protein was used as the immunogen for the APOB antibody. |
| UniProt ID | P04114 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application notes | Optimal dilution of the APOB antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | APOB/4332 |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Hazard Information | This APOB antibody is available for research use only. |
| Note | For research use only |
IHC staining of FFPE human liver tissue with APOB antibody (clone APOB/4332). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free APOB antibody (clone APOB/4332) as confirmation of integrity and purity.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using APOB antibody (clone APOB/4332). These results demonstrate the foremost specificity of the APOB/4332 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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