APH1A Antibody

• Catalog Number: orb1247790
• Category: Antibodies
• Description: APH1A Antibody
• Target: APH1A
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HVTDRSDARLQYG
• UniProt ID: Q96BI3
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 26.8kDa according to NP_057106.2). Recommended concentration: 0.05-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APH1A, anterior pharynx defective 1 homolog A (C.
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_057106.2, NP_001071096.1

orb1247790 (0.05 ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247790 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247790 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.