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APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)
Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 14688 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35 ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.

APG8b (MAP1LC3B) -T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).

APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Protocol Information
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (orb1933548)
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