APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)

SKU: orb1933548

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-106 amino acids from the Central region of human APG8b (MAP1LC3B)-T93/Y99. Antigen Region: 74-106 aa.
• Target: MAP1LC3B (HGNC:13352)
• Molecular Weight: 14688 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35 ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.

APG8b (MAP1LC3B) -T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).

APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B) -T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

MAP1LC3B (HGNC:13352)

UniProt

Q9GZQ8

RefSeq (Protein):NP_073729.1