Apg7/ATG7 Antibody

• Catalog Number: orb1537757
• Category: Antibodies
• Description: Apg7/ATG7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A 17 AA synthetic peptide near the N-Terminus of human APG7. The immunogen is located within the first 50 AA of APG7.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: Apg7 / ATG7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: ATG7, Apg7, APG7L, APG7-LIKE, Autophagy related 7,
• Note: For research use only
• Application notes: Further information: APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 77 kDa Observed: 77 kDa
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Western blot of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.