APEX2 Antibody (Center)

• Catalog Number: orb1928177
• Category: Antibodies
• Description: APEX2 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBZ4
• MW: 57401 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: APE2, APEXL2, XTH2
• Research Area: Cell Biology
• Note: For research use only

APEX2 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-APEX2 Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APEX2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescent analysis of U251 cells, using APEX2 Antibody (Center). Diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).