APEX1 Antibody

SKU: orb1787887

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: IF: 1:25, WB: 0.25, IHC: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,Κ
• Immunogen: This APEX1 antibody is generated from a mouse immunized with a recombinant protein of human APEX1. Antigen Region: 1-318 aa.
• Target: APEX1
• Molecular Weight: 35555 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-(apurinic or apyrimidinic site) lyase, 31--, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1

All lanes: Anti-APEX1 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining APEX1 in human stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

Quick Database Links

Gene Symbol

APEX1

UniProt

P27695