AP3D1 Antibody

SKU: orb345296

Description

AP3D1 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to AP3D1 by western blotting in the appropriate cell lysate or extract. Multiple isoforms exist for this protein.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1.
• Target: AP3D1
• Purity: This affinity purified antibody is directed against human AP3D1. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and chicken based on 100% homology for the immunogen sequence. Cross reactivity with AP3D1 protein from mouse, rat, dog and cow may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP3D1 proteins from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.6 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-AP3D1 antibody, AP-3 complex subunit delta-1, Adapter-related protein complex 3 subunit delta-1, AP-3 complex subunit delta, Delta-adaptin

Western blot using Biorbyt's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate (p/n orb348668). The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG (p/n orb346990) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 30-sec exposure time.

Quick Database Links

Gene Symbol

AP3D1

UniProt

O14617

RefSeq:117553580