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Catalog Number | orb345296 |
---|---|
Category | Antibodies |
Description | AP3D1 antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1. |
Concentration | 1.6 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human AP3D1. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and chicken based on 100% homology for the immunogen sequence. Cross reactivity with AP3D1 protein from mouse, rat, dog and cow may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP3D1 proteins from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | O14617 |
NCBI | 117553580 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-AP3D1 antibody, AP-3 complex subunit del Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to AP3D1 by western blotting in the appropriate cell lysate or extract. Multiple isoforms exist for this protein. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HeLa whole cell lysate using AP3D1 antibody
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