AP2B1 Antibody

• Catalog Number: orb1258348
• Category: Antibodies
• Description: AP2B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 105kDa
• Target: AP2B1
• UniProt ID: P63010
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AP2B1, ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using AP2B1 antibody (orb1258348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (orb1258348) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (orb1258348) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (orb1258348) at dilution of 1:100 (40x lens).