AP2A1/AP2-Alpha Antibody

• Catalog Number: orb1539391
• Category: Antibodies
• Description: AP2A1/AP2-Alpha Antibody
• Target: AP2A1 / AP2-Alpha
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Equine, Human, Monkey, Mouse, Porcine, Rabbit
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
• Tested applications: ELISA, ICC, IHC, IHC-P, WB
• Dilution range: ICC, IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, A
• Note: For research use only

AP2A1 antibody (0.1 ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594.

AP2A1 antibody (0.1 ug/ml) staining of Mouse Brain lysate (~5 ug protein in SDS PAGE buffer).