ANXA2 Antibody (N-term)

• Catalog Number: orb1929277
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15421
• Tested applications: IHC-P, WB
• Predicted Reactivity: Porcine, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 38604 Da
• Target: This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
• UniProt ID: P07355
• NCBI: NP_004030.1, NP_001002857.1, NP_001002858.1, NP_001129487.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Annexin A2, Annexin II, Annexin-2, Calpactin I hea
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-ANXA2 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). ANXA2 (arrow) was detected using the purified Pab.

ANXA2 Antibody (N-term) western blot analysis in 293, MDA-MB231 cell line lysates (35 ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow).

Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ANXA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: SH-SY5Y whole cell lysates. Lane 5: SK-BR-3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.