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Description
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1-500-1-1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZC3H11A. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ZC3H11A Antibody (N-term) [orb1927502]
WB
Gallus
Human, Mouse
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μlZC3H11A Rabbit Polyclonal Antibody [orb77680]
ELISA, IHC, WB
Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
100 μg

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Western blot analysis of ZC3H11A expression in Ramos (A), mouse spleen (B), H9C2 (C) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 89 kD)

Immunofluorescent analysis of ZC3H11A staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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UniProt Details
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Protocol Information
ZC3H11A Antibody (orb214940)
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