ZAP70 Mouse Monoclonal Antibody

SKU: orb705545

Description

The ZAP70 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within human ZAP70. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ZAP70. The exact sequence is proprietary.
• Target: ZAP70
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Western blot analysis of ZAP70 expression in JurKat (A) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 69 kD)

Immunohistochemical analysis of ZAP70 staining in human hodgkin's lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

ZAP70

UniProt

P43403

NCBI Gene ID

7535