XRCC4 Mouse Monoclonal Antibody

SKU: orb323202

Description

The XRCC4 Antibody is suitable for IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human XRCC4. The exact sequence is proprietary. Purification: Affinity chromatography

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500, IP: 1:100-1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human XRCC4. The exact sequence is proprietary.
• Target: XRCC4
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA repair protein XRCC4; X-ray repair cross-complementing protein 4

Western blot analysis of XRCC4 expression in Hela (A), 293T (B) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

Immunohistochemical analysis of XRCC4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of XRCC4 from 0.5 mg Hela whole cell extract lysate, using Anti-XRCC4 Antibody.

Quick Database Links

Gene Symbol

XRCC4

UniProt

Q13426

NCBI Gene ID

7518