XIAP Mouse Monoclonal Antibody

SKU: orb1184736

Description

Anti-XIAP Antibody (monoclonal, 3G2G1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Disease Biomarkers, Immunology & Inflammation, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 3G2G1
• Immunogen: E.coli-derived human XIAP recombinant protein (Position: A15-V244). Human XIAP shares 89.4% and 90.7% amino acid (aa) sequence identity with mouse and rat XIAP, respectively.
• Target: E3 ubiquitin-protein ligase XIAP
• Molecular Weight: 54 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

API3; BIRC4; hIAP 3; hIAP3; hILP; IAP 3; IAP like protein; IAP3; ILP; ILP1; MIHA; X linked IAP; XIAP; XLP2

Western blot analysis of XIAP using anti-XIAP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XIAP antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for XIAP at approximately 54 kDa. The expected band size for XIAP is at 57 kDa.

Quick Database Links

Gene Symbol

E3 ubiquitin-protein ligase XIAP

UniProt

P98170