XAF1 Antibody

• Catalog Number: orb1879763
• Category: Antibodies
• Description: Rabbit polyclonal antibody to XAF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human XAF1. The exact sequence is proprietary.
• UniProt ID: Q6GPH4
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BIRC4BP; XIAPAF1; XIAP-associated factor 1; BIRC4-
• Note: For research use only
• Entrez: 54739
• Expiration Date: 12 months from date of receipt.

Western blot analysis of XAF1 expression in human liver (A), human spleen (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 32 kD)

Immunofluorescent analysis of Anti-XAF1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).