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Catalog Number | orb1879763 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to XAF1 |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human XAF1. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/10 - 1/50) |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Entrez | 54739 |
UniProt ID | Q6GPH4 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Alternative names | BIRC4BP; XIAPAF1; XIAP-associated factor 1; BIRC4- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of XAF1 expression in human liver (A), human spleen (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 32 kD)
Immunofluorescent analysis of Anti-XAF1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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