Wnt2b Monoclonal Antibody

SKU: orb548331

Description

Anti-Wnt2b Monoclonal Antibody. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-2000ICC/IF 1:50-200FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AEED-23
• Immunogen: A synthesized peptide derived from human Wnt2b Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Molecular Weight: 60-85 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Thymidine kinase, cytosolic; 2.7.1.21; TK1

Western blot analysis of WNT2B expression in HepG2 cell lysate using anti-WNT2B antibody (orb548331). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2B antigen affinity purified polyclonal antibody (Catalog # orb548331) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for WNT2B.

Quick Database Links

UniProt

Q93097