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Anti-Visfatin Antibody

Catalog Number: orb1474417

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1474417
CategoryAntibodies
DescriptionRabbit monoclonal antibody to Visfatin
Species/HostRabbit
ClonalityMonoclonal
Tested applicationsIF, IH, IP, WB
ReactivityHuman, Mouse, Rat
ImmunogenA synthetic peptide of human Visfatin
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
ConjugationUnconjugated
TargetNAMPT
Entrez10135, 59027, 297508
UniProt IDQ99KQ4, P43490, Q80Z29
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Alternative namesPBEF; PBEF1; Nicotinamide phosphoribosyltransferas
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Visfatin Antibody

Western blot analysis of Visfatin expression in NIH3T3 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 56 kD)

Anti-Visfatin Antibody

Immunohistochemical analysis of Visfatin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.101). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Visfatin Antibody

Immunofluorescent analysis of Visfatin staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

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