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Anti-VIP peptides VIP Antibody

Catalog Number: orb371644

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb371644
CategoryAntibodies
DescriptionAnti-VIP peptides VIP Antibody
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human VIP, different from the related mouse and rat sequences by four amino acids.
UniProt IDP01282
MW26 kDa
Tested applicationsIF, IHC
Application notesImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunofluorescence, 5 μg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesVIP peptides; Intestinal peptide PHV-42; Peptide h
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NoteFor research use only
Anti-VIP peptides VIP Antibody

IF analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-VIP Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-VIP peptides VIP Antibody

IHC analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VIP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-VIP peptides VIP Antibody

IHC analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VIP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-VIP peptides VIP Antibody

IHC analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VIP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-VIP peptides VIP Antibody

IHC analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VIP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-VIP peptides VIP Antibody

IHC analysis of VIP using anti-VIP antibody. VIP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VIP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

  • VIP rabbit pAb [orb770383]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Anti-VIP Antibody [orb339202]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 200 μl, 100 μl
  • VIP peptide [orb339596]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • VIP Antibody [orb674632]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg
  • Anti-VIP peptides VIP Antibody [orb2617844]

    IF,  IHC

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg