Anti-VEGF [A.4.6.1]

• Catalog Number: orb348842
• Category: Antibodies
• Description: Mouse monoclonal antibody to VEGF
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A.4.6.1
• Tested applications: Blocking, ELISA, FC, IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG1
• Immunogen: Recombinant human VEGF.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: VEGF
• UniProt ID: P15692
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VEGF-A; Vascular Endothelial Growth Factor; VEGFA;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Kim, K. J. et al. The vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies Growth Factors, 7, 53-64 (1992)

PubMed: 1380254

Immunohistochemical staining of rat brain tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat brain tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 (orb348842) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat skin tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat skin tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 (orb348842) was used to stain samples at a concentration of 5 µg/ml.

Western Blot using anti-VEGF antibody. Rat skin lysate was resolved on a 10% SDS PAGE gel and blots probed with orb348842 at 1.0 µg/ml before being detected by a secondary antibody. The expected band size (27 kDa) is seen along with bands corresponding to dimers.