VCP Antibody (FITC)

SKU: orb43875

Description

Mouse Monoclonal to Intra-Acrosomal Protein.

Research Area

Autophagic, Cancer, DNA Damage, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Mouse
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: Hs-14
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Target: VCP
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TERA, CDC48

Separation of human sperm cells stained using anti-VCP (Hs-14) FITC antibody (concentration in sample 3 µg/ml, red) from unstained human sperm cells (black) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

VCP

UniProt

P55072

NCBI Gene ID

7415