Anti-USF2 Antibody

• Catalog Number: orb214720
• Category: Antibodies
• Description: Rabbit polyclonal antibody to USF2
• Target: USF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.
• UniProt ID: Q63665, Q15853, Q64705
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BHLHB12 antibody, anti Upstream stimulatory f
• Note: For research use only
• Entrez: 81817, 7392, 22282

Western blot analysis of USF2 expression in Hela (A), U2OS (B), mouse lung (C), mouse testis (D), rat lung (E) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 44 kD)

Immunohistochemical analysis of USF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.