Uroplakin3a UPK3A Rabbit Monoclonal Antibody

SKU: orb548418

Description

Anti-Uroplakin3a UPK3A Monoclonal Antibody. Tested in IHC, Flow Cytometry applications. This antibody reacts with Human.

Research Area

Cancer Biology, Disease Biomarkers

Images & Validation

• Tested Applications: FC, IHC
• Dilution Range: IHC 1:1000-1:500 FC 1:100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFEH-21
• Immunogen: A synthesized peptide derived from human Uroplakin III Component of the asymmetric unit membrane (AUM) ; a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells.
• Target: Uroplakin-3a
• Molecular Weight: 23 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Death domain-containing protein CRADD; Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD; RAIDD

IHC analysis of UPK3A using anti-UPK3A antibody (orb548418) with paraffin-embedded human bladder tissue. UPK3A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UPK3A Antibody (orb548418) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Uroplakin-3a

UniProt

O75631