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Anti-UBE2F Antibody

Catalog Number: orb412329

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb412329
CategoryAntibodies
DescriptionRabbit polyclonal antibody to UBE2F
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human UBE2F
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetUBE2F
Entrez140739, 363284
UniProt IDQ969M7, Q5U203
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-NCE2 antibody, anti-NEDD8-conjugating enzyme
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-UBE2F Antibody

Western blot analysis of UBE2F expression in K562 (A), mouse liver (B), mouse heart (C) whole cell lysates. (Predicted band size: 11; 13; 17; 18; 21; 23 kD; Observed band size: 21 kD)

Anti-UBE2F Antibody

Immunohistochemical analysis of UBE2F staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-UBE2F Antibody

Immunofluorescent analysis of UBE2F staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    ELISA,  IHC,  WB

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    Goat

    Polyclonal

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    100 μg
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    ELISA,  WB

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    100 μg
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    ELISA

    Bovine, Canine, Human, Mouse, Porcine, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
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    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl