Anti-TXNRD1 Antibody

• Catalog Number: orb668546
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TXNRD1
• Target: TXNRD1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human TXNRD1. The exact sequence is proprietary.
• UniProt ID: O89049, Q16881, Q9JMH6
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-GRIM12 antibody, anti-KDRF antibody, anti-Thi
• Note: For research use only
• Entrez: 50493, 7296, 58819

Western blot analysis of TXNRD1 expression in HepG2 (A), mouse heart (B) whole cell lysates. (Predicted band size: 50; 54; 59; 60; 65; 67; 70 kD; Observed band size: 65 kD)

Immunohistochemical analysis of TXNRD1 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TXNRD1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).