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Item 1 of 3
Anti-TSC2 (Phospho-T1462) Antibody
Catalog Number: orb224038
| Catalog Number | orb224038 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to TSC2 (Phospho-T1462) |
| Target | TSC2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Primate, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T1462 of human TSC2 protein. The exact sequence is proprietary. |
| UniProt ID | P49816, Q61037, P49815 |
| Tested applications | IF, IH, WB |
| Dilution range | WB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti TSC4 antibody, anti Tuberin antibody, anti Tu Read more... |
| Note | For research use only |
| Entrez | 24855, 7249 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of TSC2 (Phospho-T1462) expression in SHSY5Y (A), PC3 (B), LNCaP (C), NIH3T3 (D), PC12 (E) whole cell lysates. (Predicted band size: 200 kD; Observed band size: 200 kD)
Immunohistochemical analysis of TSC2 (Phospho-T1462) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TSC2 (Phospho-T1462) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
