Anti-Trop2 (sacituzumab biosimilar) mAb

• Catalog Number: orb689328
• Category: Antibodies
• Description: Anti-Trop2 (sacituzumab biosimilar) mAb
• Species/Host: Human Humanized
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Biosimilar Antibody
• Dilution range: ELISA 1:5000-10000; Flow Cyt 1:100
• Form/Appearance: Powder
• Purity: Purified from cell culture supernatant by affinity chromatography
• Conjugation: Unconjugated
• Target: Trop2
• UniProt ID: P09758
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
• Alternative names: TACSTD2;GA733-1;M1S1;TROP2
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human Trop2, mFc-His tagged protein orb689477 can bind Anti-Trop2 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.

Flow cytometry analysis of antigen binding of anti-human Trop2 mAb (orb689328). (A) orb689328 does not bind to 293T cells that do not express Trop2. (B) A clear peak shift of orb689328 was seen compared to the control when incubated with Trop2-expressing A431 cells, indicating strong binding of orb689328 to Trop2. Antibodies were incubated at 2 μg/mL.

HEK293 cell line transfected with irrelevant protein (B) and human Trop2 protein (A) were surface stained with anti-Trop2 neutralizing antibody 1 μg/ml (sacituzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.