TRIM Antibody

SKU: orb44526

Description

Mouse Monoclonal to TRIM.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Western blotting: Non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: TRIM-04
• Immunogen: Recombinant intracellular domain (aa 29-186) of human TRIM.
• Target: TRIM
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRAT, TCRIM

Western blotting analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb TRIM-04 and goat anti-mouse IgG (H+L)-HRP conjugate.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 positive TRIM positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Quick Database Links

Gene Symbol

TRIM

UniProt

Q6PIZ9

NCBI Gene ID

50852